MEF-1

98061101, mouse embryo, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• product name: MEF-1, 98061101
• 생물학적 소스: mouse embryo
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse embryo fibroblast, SV40 - transformed

세포주 설명

The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (Sigma Catalogue number. 97040310) and PEA13 (Sigma Catalogue number. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.

애플리케이션

Study of LRP and LRP-deficiency

배양 배지

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

기타 정보

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