EMT6/AR10.0

96042326, mouse unknown/unspecified, Epithelial-like

• UNSPSC 코드: 41106514

속성

• product name: EMT6/AR10.0, 96042326
• 생물학적 소스: mouse unknown/unspecified
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse mammary, drug-resistant

세포주 설명

The drug resistant cell line EMT6/AR10.0 was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by continuous growth in increasing concentrations of doxorubicin. It was reported to show reduced intracellular drug accumulation and hyperexpression of P-glycoprotein. It is recommended to culture the cells without drug after resuscitation until the first passage.

애플리케이션

Drug-resistance studies

배양 배지

MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA)+ 10.0μg/ml doxorubicin + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5%CO₂; 37ºC; resuscitate in drug-free media, add doxorubicin after first passage

기타 정보

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