PNT1A human

95012614, human prostate, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: PNT1A human, 95012614, Normal prostate epithelium imortalized with SV40.
• 생물학적 소스: human prostate
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Dihydrotestosterone receptor
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

세포주 기원

Human post pubertal prostate normal, immortalised with SV40

세포주 설명

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.

애플리케이션

PNT1A has been used to determine the variation in metabolite levels of human DU145 prostate cancer and PNT1A cells after treatment with selenomethionine (SeM) and Se-methylselenocysteine (SeMSC). It has also been used to study the effects of angiotensin II (Ang II) and relaxin 2 (RLN2) on the viability, proliferation, adhesion, migration and invasion of PNT1A cells.

Study of proliferation, oncogene activation and differentiation of adult prostatic cells and initial steps leading to transformation.

배양 배지

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37°C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.