ATHOS

93122325, Porcine thyroid, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: ATHOS, 93122325
• 생물학적 소스: Porcine thyroid
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Thyroglobulin, plasminogen activator (u-PA)
• 수용체: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Porcine thyroid epithelial

세포주 설명

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

애플리케이션

Mechanism of thyroid follicle reconstruction

배양 배지

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

기타 정보

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