MG-63 Cell Line human

86051601, human bone, Fibroblast

• 동의어(들): MG63 Cells
• UNSPSC 코드: 41106514

속성

• product name: MG-63 Cell Line human, 86051601
• 생물학적 소스: human bone
• 설명: Human osteosarcoma
• 성장 모드: Adherent
• 핵형: Modal no. 66; hypotriploid
• 형태학: Fibroblast
• 제품: Inducible interferon
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human osteosarcoma

세포주 설명

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

애플리케이션

Human MG-63 cell line has been used:

• to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells
• to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells
• as a positive control for immunostaining procedure
• to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells

Interferon-induction studies

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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