Ethyl nitrosourea induced tumour cell line. Glial cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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