3T3 Swiss Albino

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022108, mouse embryo, Fibroblast

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse embryo
• 포장: tube of 5 μg 85022108-DNA-5UG; pkg of vial of cells 85022108-1VL
• 성장 모드: Adherent
• 핵형: 2n = 40; hypertriploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

일반 설명

Product has lost contact inhibition

세포주 기원

Mouse Swiss Albino embryo fibroblast

세포주 설명

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

애플리케이션

Contact inhibition and virus studies: polyoma and SV40 viruses

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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