IIC9 (CH0)

30814, hamster embryo, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: IIC9 (CH0), 00030814
• 생물학적 소스: hamster embryo
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Chinese hamster embryo fibroblast

세포주 설명

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.

애플리케이션

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.

배양 배지

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

기타 정보

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