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Merck
모든 사진(1)

주요 문서

CA200

Sigma-Aldrich

cAMP Enzyme Immunoassay Kit, Direct

동의어(들):

cAMP detection kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84
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기술

ELISA: suitable

Quality Level

배송 상태

wet ice

저장 온도

−20°C

일반 설명

Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.[1]
Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.

애플리케이션

Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium[2] virulent and attenuated macrophages.[3] It has also been used to quantify cAMP synthesis in olfactory epithelium.[4]

생화학적/생리학적 작용

Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.[1]

기타 정보

Allow reagents to warm at room temperature for 30 minutes prior to opening.

키트 구성품 전용

제품 번호
설명

  • 0.1 M Hydrochloric Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Direct cAMP Assay Layout Sheet 1 x 1

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • Neutralizing Reagent 1 x 6

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

모두 보기 (14)

픽토그램

FlameSkull and crossbonesCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

표적 기관

Respiratory system

보충제 위험성

Storage Class Code

3 - Flammable liquids

Flash Point (°F)

120.2 °F

Flash Point (°C)

49 °C


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