C9282

Sodium cholate hydrate

BioReagent, suitable for cell culture

• 동의어(들): 3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid sodium salt, Cholalic acid sodium salt
• 실험식(Hill 표기법): C₂₄H₃₉NaO₅ · xH₂O
• CAS Number: 206986-87-0
• Molecular Weight: 430.55 (anhydrous basis)
• Beilstein: 3582354
• EC Number: 206-643-5
• MDL number: MFCD00150749
• UNSPSC 코드: 12161902
• PubChem Substance ID: 24893147
• NACRES: NA.75

속성

• 생물학적 소스: bovine bile; ovine bile
• Quality Level: 200
• 설명: anionic
• 제품 라인: BioReagent
• 분석: ≥99.0% (HPLC)
• 양식: powder
• 분자량: micellar avg mol wt 900-1300
• 응집 번호: 2-3
• 기술: cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• solubility: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• SMILES string: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1
• InChI key: MUVVIYFKOVLQHL-RCVKHMDESA-M

설명

일반 설명

Cholate is a primary bile acid[1] produced from cholesterol in the liver.[2] It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).[3]

애플리케이션

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system[4],
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor[5]

생화학적/생리학적 작용

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.[2]

안전성 정보

• 유해 및 위험 성명서: H412
• 예방조치 성명서: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)