C8482

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• 동의어(들): Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
• MDL number: MFCD03458215
• UNSPSC 코드: 41106305
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: >90% (SDS-PAGE)
• 형태: buffered aqueous glycerol solution
• 특이 활성도: >1000 units/mg protein
• 분자량: 43.5 kDa
• UniProt 수납 번호: P17612
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PRKACA(5566)

설명

생화학적/생리학적 작용

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

물리적 형태

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

분석 메모

One unit will transfer 1 nanomole of phosphate from ³²P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
K_m (ATP) = 25 μM at 30 °C, pH 7.4. K_m (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable