This product contains EDTA as the major active component, which acts as a chelating agent for divalent cations to loosen cell–cell contacts but does not break down the extracellular matrix as trypsin does. Empirical testing is needed to determine if this approach is more effective. It is highly recommended to review the literature to determine if an EDTA-only dissociation method has been successfully used with the particular primary microglia preparation protocol.
추천 제품
Quality Level
무균
sterile-filtered
제품 라인
BioReagent
양식
solution
농도
1 ×
기술
cell culture | mammalian: suitable
pH
6.5-7.5
배송 상태
wet ice
저장 온도
room temp
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Cell Dissociation Solution Non-enzymatic buffer systems are alternative to the canonical trypsin based buffers for dissociation of adherent mesenchymal stem cell (MSC) monolayers.[1]
애플리케이션
Cell Dissociation Solution Non-enzymatic 1x has been used in the dissociation of murine macrophage (RAW2647) cells,[2] A549 pulmonary cells,[3] islets cells[4] and 2D cell and 3D mammospheres.[5]
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
이미 열람한 고객
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Introduction: Proteinuria contributes to progression of renal damage, partly by complement activation on proximal tubular epithelial cells. By pattern recognition, properdin has shown to bind to heparan sulfate proteoglycans on tubular epithelium and can initiate the alternative complement pathway (AP).
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Dysfunctions of neural stem cells (NSCs) often lead to a variety of neurological diseases. Thus, therapies based on NSCs have gained increasing attention recently. It has been documented that microRNA (miR)-421 represses the autophagy and apoptosis of mouse hippocampal neurons
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Oak PS, et al.
International Journal of Cancer. Journal International Du Cancer, 131(12), 2808-2819 (2012)
Evidence for lipopolysaccharide induced differentiation of RAW264. 7 murine macrophage cell line into dendritic like cells
Saxena RK, et al.
Journal of Biosciences, 28(1), 129-134 (2003)
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Hi, I am working with primary microglia and it very tricky to dissociate. I usually dissociate them with 0.5% Trypsin for 7 min at 37˚C and still hard to collect them. May I know whether this reagent would be a better alternative? Thank you!
1 답변-
도움이 되었습니까?
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What is the concentration of EDTA in Cat#C5789? Thanks
1 답변-
The concentration of EDTA in this formulation is proprietary. Additional components include Sodium Citrate, Nitrilotriacetic Acid, and Glycerol in Hank's Balanced Salt solution. Should the concentration of EDTA be required, product E8008, EDTA 0.2% in DPBS, may be of interest. Both items are tested for cell dissociation. Please see the link below to review this product option:
https://www.sigmaaldrich.com/US/en/product/sigma/e8008도움이 되었습니까?
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