추천 제품
생물학적 소스
Aspergillus niger
양식
ammonium sulfate suspension
특이 활성도
≥4,000 units/mg protein
분자량
tetramer ~250 kDa
저장 조건
(Tightly closed)
기술
FISH: suitable
배송 상태
wet ice
저장 온도
2-8°C
SMILES string
O(CC)C(=O)c1ccc(cc1)O
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI key
NUVBSKCKDOMJSU-UHFFFAOYSA-N
일반 설명
애플리케이션
Catalase from Aspergillus niger has been used:
- as a positive control during the functional characterization of Clostridium difficile spore coat proteins.[4]
- as a component of the catalase solution to prepare GLOX buffer with enzymes to maintain the embryos of Caenorhabditis elegans before single-molecule fluorescence in situ hybridization (smFISH) studies[5]
- as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of platelet-rich plasma[6]
- as a supplement in Todd Hewitt media plus 0.5 % yeast extract (THY) media for the neutralization of pneumococcal H2O2[7]
생화학적/생리학적 작용
Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.
Catalase is involved in catalyzing the degradation of hydrogen peroxide (H2O2) to water and free oxygen. Catalase is used commercially for decomposing H2O2 as it is added as an antimicrobial agent in process streams.[8] It also catalyzes the oxidation of electron donors like ethanol and phenols during lower concentrations of H2O2.[9] Catalase shows antioxidant activity by protecting cells from higher levels of reactive oxygen species (ROS). Increased expression of catalase is observed in chronic lymphocytic leukemia, gastric cancer, glioma, and melanoma. Decreased levels of catalase are seen in acute myeloid leukemia, lung, pancreatic, prostate, and skin (non-melanoma) cancers. Hence catalase exhibits a dual role in cancer.[2]
주의사항
Freezing of solutions is not recommended.
단위 정의
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
물리적 형태
Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
분석 메모
Protein determined by biuret
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Seleipiri Charles et al.
Analytical chemistry, 93(3), 1369-1376 (2020-12-24)
Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and
3D structure modeling of catalase enzyme from Aspergillus Fumigatus
Dwivedi V D, et al.
Open journal of immunology, 1(1) (2016)
J Fiedurek et al.
Journal of applied microbiology, 89(1), 85-89 (2000-08-17)
A novel method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide (H2O2) to the medium which is then decomposed to oxygen and water by catalase. Some factors affecting oxygenation of culture were
L Brunelli et al.
Free radical biology & medicine, 30(7), 709-714 (2001-03-29)
Previously, we found that catalase enhanced the protection afforded by superoxide dismutase to Escherichia coli against the simultaneous generation of superoxide and nitric oxide (Brunelli et al., Arch. Biochem. Biophys. 316:327-334, 1995). Hydrogen peroxide itself was not toxic in this
Joseph C Bryant et al.
BMC microbiology, 16(1), 271-271 (2016-11-11)
Streptococcus pneumoniae is one of the leading causes of community acquired pneumonia and acute otitis media. Certain aspects of S. pneumoniae's virulence are dependent upon expression and release of the protein toxin pneumolysin (PLY) and upon the activity of the
문서
Instructions for working with enzymes supplied as ammonium sulfate suspensions
프로토콜
This procedure may be used for all Catalase products.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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