This item is a product of PromoCell. The manufacturer considers the formulation proprietary. However, this is a complete medium that includes all the growth factors and supplements necessary for providing optimal cell health and efficient adherence selection of monocytes from freshly isolated mononuclear cells. This medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
제품 C-28051은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.
추천 제품
양식
aqueous solution
포장
pkg of 250 ml
기술
cell culture | mammalian: suitable
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
Monocyte Attachment Medium (Ready-to-use)
애플리케이션
The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is dispensable prior to experiments requiring adherent monocytes as a starting material. With properly performed washing steps large numbers of 80-90% pure monocytes can be obtained. Typical applications are generation of dendritic cells / macrophages as well as phagocytosis assays.Recommended for:Human mononuclear cells (freshly isolated)Human monocytes (freshly isolated)
품질
All lots of PromoCell Monocyte Attachment Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from peripheral blood. Each lot of Monocyte Attachment Medium is checked for the efficient adherence selection of highly viable monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
제조 메모
The Medium is chemically defined/xeno-free and does not need further supplementation.
저장 및 안정성
Store the Medium at 2 to 8°C in the dark immediately after arrival. Do not freeze the Medium. If stored properly, the product is stable until the expiry date stated on the label. After opening, the shelf life of the medium is 6 weeks at 2 to 8°C. For use, pre-warm only an aliquot of the medium and keep the remaining medium refrigerated at 2 to 8°C.
계대배양 정규 작업
Click here for more information.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
프로토콜
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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