C2366

Anti-K⁺/Cl^- Cotransporter (KCC2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): Anti-DEE34, Anti-EIEE34, Anti-EIG14, Anti-KCC2, Anti-hKCC2
• MDL number: MFCD06798467
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 종 반응성: canine, rat
• 기술: western blot: 0.5-2.0 μg/mL using rat brain membrane preparation
• UniProt 수납 번호: Q63633
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: rat ... Slc12a5(171373)

설명

일반 설명

Transporters and exchangers play a critical role in the generation and dissipation of action potentials in nerve cells and in the maintenance of normal cell volume. They are also involved in a variety of mechanisms pertaining to the control of neuronal growth, maturation, synaptic plasticity and neuroendocrine functions
The cation-chloride cotransporters (CCCs) are glycoproteins involved in transport of ions, including chloride, across the cell membrane without an accompanying net charge movement; thus this type of transport is driven without the direct hydrolysis of ATP. The energy for the transport is derived from the cation gradient generated by the Na⁺/K⁺/ATPase. The CCCs also play a critical role in influencing GABA- and glycine-mediated signaling. Other transporters that participate in chloride homeostasis include Na⁺-dependent and Na⁺-independent anion exchangers, which exchange chloride for HCO3^- ions
Three cotransporters belonging to this family include sodiumchloride( Na⁺/Cl^-/ ) cotransporters (NCCs), sodiumpotassium-chloride (Na⁺/K⁺/2Cl^-) cotransporters (NKCCs) and potassium-chloride (K⁺/Cl^-) cotransporters (KCCs). K⁺/Cl^- cotransporter (KCC2) is the major neuronal chloride transporter. It has been proposed to play a role in the modulation of neuronal responses to γ-aminobutyric acid (GABA). GABA-mediated fasthyperpolarizing inhibition depends on extrusion of chloride by KCC2 In conditions of neuronal damage, where neuronal excitability is increased, and in spinal cord in models of neuropathic pain, the expression of the KCC2 transporter is decreased

특이성

Anti-K⁺/Cl^- Cotransporter (KCC2) specifically recognizes K⁺/Cl^- cotransporter. Wide range of species cross-reactivity expected due to sequence homology.

Wide range of species cross-reactivity expected due to sequence homology.

면역원

N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K⁺/Cl^- Cotransporter (KCC2).

애플리케이션

Anti-K⁺/Cl^- Cotransporter (KCC2) specifically recognizes K⁺/Cl^- cotransporter in rat brain membrane preparations by immunoblotting and Madin Darby Canine Kidney (MDCK) cells by immunocytochemistry.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

표적 설명

Anti-K⁺/Cl^- Cotransporter (KCC2) specifically recognizes K⁺/Cl^- cotransporter (KCC2). The cation-chloride cotransporters (CCCs) are glycoproteins involved in transport of ions, includingchloride, across the ce

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)