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Merck
모든 사진(1)

문서

C-21060

Sigma-Aldrich

Airway Epithelial Cell Growth Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

동의어(들):

Primary Cell Culture Media

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

형태

aqueous solution

포장

pkg of 500 ml

기술

cell culture | mammalian: suitable

배송 상태

wet ice

일반 설명

Airway Epithelial Cell Growth Medium (Ready-to-use)

애플리케이션

Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages. The medium is optimized for primary human cells, but can also be used for equine, porcine, murine, bat and rat airway epithelial cells.Recommended for:Human Nasal Epithelial Cells (HNEpC)Human Bronchial Epithelial Cells (HBEpC)Human Tracheal Epithelial Cells (HTEpC)

성분

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

품질

All lots of PromoCell Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested airway epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

제조 메모

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 6.7 ng / mlTransferrin, holo (human): 10 µg / mlRetinoic Acid: 0.1 ng / ml

저장 및 안정성

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

계대배양 정규 작업

Click here for more information.

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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문서 라이브러리 방문

문서

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

관련 콘텐츠

생물학적 시스템을 정확하게 복제하기 위한 인간 일차 세포주와 관련된 전문적 프로토콜 및 배양 배지에 대해 알아보세요.

Explore expert protocols and culture media for human primary cell lines from PromoCell® and Sigma-Aldrich. Closely replicate biological systems with authenticated human primary cells, blood and stem cells, cancer cells, and tumor spheroid cultures.

Explore expert protocols and culture media for human primary cell lines to replicate biological systems accurately.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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