synthetic peptide corresponding to amino acids 718-732 of human CPSF2 conjugated to KLH. The corresponding sequence is identical in mouse.
애플리케이션
Anti-CPSF2 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 2-5μg using A20 cell lysates and western blotting at a concentration of 2-4μg/mL using K562 cell lysates.
생화학적/생리학적 작용
Cleavage and polyadenylation specificity factor subunit 2 is a protein encoded by the CPSF2 gene in humans. It is also referred to as CPSF100. CPSF and cleavage stimulation factor (CstF) play an important role during the formation of the 3′ ends of mRNA for 3′ cleavage of the transcript as well as for subsequent polyadenylation. CPSF and poly(A) polymerase (PAP) activity is controlled by Eg2-catalyzed phosphorylation during formation of cytoplasmic polyadenylation complex.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The cleavage stimulation factor (CstF), and the cleavage and polyadenylation specificity factor (CPSF) are necessary for 3'-terminal processing of polyadenylated mRNAs. To study the distribution of 3' cleavage factors in the nuclei of human T24 cells, monoclonal antibodies against the
Molecular and cellular biology, 14(12), 8183-8190 (1994-12-01)
During the formation of the 3' ends of mRNA, the cleavage and polyadenylation specificity factor (CPSF) is required for 3' cleavage of the transcript as well as for subsequent polyadenylation. Using peptide sequences from a tryptic digest, we have cloned
Development (Cambridge, England), 128(14), 2815-2822 (2001-08-30)
In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB, a sequence-specific RNA binding
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