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Merck
모든 사진(1)

주요 문서

C1872

Sigma-Aldrich

Anti-CPSF2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-100-KD subunit, Anti-CPSF100, Anti-Cleavage and polyadenylation specificity factor 2

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About This Item

UNSPSC 코드:
12352203

생물학적 소스

rabbit

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

양식

buffered aqueous solution

분자량

antigen ~100 kDa

종 반응성

human

농도

~1.0 mg/mL

기술

immunoprecipitation (IP): 2-5 μg using A20 cell lysates
western blot: 2-4 μg/mL using K562 cell lysates

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

유전자 정보

human ... CPSF2(53981)
mouse ... Cpsf2(51786)

면역원

synthetic peptide corresponding to amino acids 718-732 of human CPSF2 conjugated to KLH. The corresponding sequence is identical in mouse.

애플리케이션

Anti-CPSF2 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 2-5μg using A20 cell lysates and western blotting at a concentration of 2-4μg/mL using K562 cell lysates.

생화학적/생리학적 작용

Cleavage and polyadenylation specificity factor subunit 2 is a protein encoded by the CPSF2 gene in humans. It is also referred to as CPSF100. CPSF and cleavage stimulation factor (CstF) play an important role during the formation of the 3′ ends of mRNA for 3′ cleavage of the transcript as well as for subsequent polyadenylation. CPSF and poly(A) polymerase (PAP) activity is controlled by Eg2-catalyzed phosphorylation during formation of cytoplasmic polyadenylation complex.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서 라이브러리 방문

W Schul et al.
The EMBO journal, 15(11), 2883-2892 (1996-06-03)
The cleavage stimulation factor (CstF), and the cleavage and polyadenylation specificity factor (CPSF) are necessary for 3'-terminal processing of polyadenylated mRNAs. To study the distribution of 3' cleavage factors in the nuclei of human T24 cells, monoclonal antibodies against the
A Jenny et al.
Molecular and cellular biology, 14(12), 8183-8190 (1994-12-01)
During the formation of the 3' ends of mRNA, the cleavage and polyadenylation specificity factor (CPSF) is required for 3' cleavage of the transcript as well as for subsequent polyadenylation. Using peptide sequences from a tryptic digest, we have cloned
R Hodgman et al.
Development (Cambridge, England), 128(14), 2815-2822 (2001-08-30)
In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB, a sequence-specific RNA binding

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