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주요 문서

C-12971

Sigma-Aldrich

Human Mesenchymal Stem Cells (hMSC)

Derived from umbilical cord matrix, 500,000 cryopreserved cells

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About This Item

UNSPSC 코드:
41106514
NACRES:
NA.81
C-12971 제품은 고객 맞춤형 제품으로 웹사이트에서 판매할 수 없습니다. 고객지원팀으로 연락바랍니다.

생물학적 소스

human umbilical cord matrix (Wharton′s jelly)

포장

pkg of 500,000 cells

형태학

(stromal)

기술

cell culture | mammalian: suitable

배송 상태

dry ice

저장 온도

−196°C

일반 설명

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

세포주 기원

Umbilical Cord

애플리케이션

Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Human Mesenchymal Stem Cells (hMSC-UC) are harvested from normal human umbilical cord matrix (Wharton′s jelly) of individual donors and are provided in a cryopreserved format. The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. The cells show a verified marker expression profile that complies with ISCT recommendations, providing well characterized cells (Cytotherapy (2006) Vol. 8, No. 4, 315-317).

품질

Rigid quality control tests are performed for each lot of Mesenchymal Stem Cells. They are tested for cell morphology, proliferation potential, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis of a comprehensive panel of markers, namely CD73/CD90/CD105 and CD14/CD19/CD34/CD45/HLA-DR as proposed by the ISCT. Differentiation assays into adipogenic, osteogenic and chrondrogenic are performed for each lot under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, and microbial contaminants (fungi, bacteria, and mycoplasma).

경고

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

계대배양 정규 작업

Click here for more information.

기타 정보

Recommended Plating Density: 4000 cells per cm2Tested Markers: CD105 positive, CD73 positive, CD90 positive, CD45 negative, CD34 negative, CD14 negative, CD19 negative, HLA-DR negativeDifferentiation capacity to adipogenic, osteogenic and chondrogenic cells

추천 제품

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

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이 제품을 이미 가지고 계십니까?

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문서 라이브러리 방문

문서

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

모두 보기

프로토콜

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

관련 콘텐츠

생물학적 시스템을 정확하게 복제하기 위한 인간 일차 세포주와 관련된 전문적 프로토콜 및 배양 배지에 대해 알아보세요.

Explore expert protocols and culture media for human primary cell lines from PromoCell® and Sigma-Aldrich. Closely replicate biological systems with authenticated human primary cells, blood and stem cells, cancer cells, and tumor spheroid cultures.

Explore expert protocols and culture media for human primary cell lines to replicate biological systems accurately.

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