B6149
5-Bromo-4-chloro-3-indolyl phosphate disodium salt
chromogenic, ≥98% (HPLC), powder
동의어(들):
5-BCIP disodium salt, BCIP®, X-phosphate disodium salt
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모든 사진(1)
About This Item
Linear Formula:
C8H4BrClNO4P · 2Na
CAS Number:
Molecular Weight:
370.43
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.83
추천 제품
product name
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, ≥98% (HPLC)
분석
≥98% (HPLC)
형태
powder
solubility
H2O: 20 mg/mL
DMF: insoluble
저장 온도
−20°C
SMILES string
[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
InChI
1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
InChI key
OAZUOCJOEUNDEK-UHFFFAOYSA-L
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애플리케이션
5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
기질
Histochemical substrate for alkaline phosphatase.
법적 정보
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Manli Qi et al.
Journal of bacteriology, 193(10), 2498-2509 (2011-03-29)
The Chlamydia-specific hypothetical protein CT795 was dominantly recognized by human antisera produced during C. trachomatis infection but not by animal antisera raised against dead chlamydia organisms. The immundominant region recognized by the human antibodies was mapped to the N-terminal fragment
P E Høyer et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(8), 855-863 (1996-08-01)
We describe a quantitative histochemical method for demonstration of five N-acetyl-glucosamine binding lectins in the syncytiotrophoblast of human term placenta. The method employs biotinylated lectins and alkaline phosphatase-conjugated avidin. The alkaline phosphatase activity is detected by using 5-bromo-4-chloro-indoxyl phosphate as
Shailesh Kumar et al.
PLoS genetics, 15(6), e1008219-e1008219 (2019-06-27)
Fes1 is a conserved armadillo repeat-containing Hsp70 nucleotide exchange factor important for growth at high temperature, proteasomal protein degradation and prion propagation. Depleting or mutating Fes1 induces a stress response and causes defects in these processes that are ascribed solely
Thermal preference ranges correlate with stable signals of universal stress markers in Lake Baikal endemic and Holarctic amphipods
Axenov-Gribanov D, et al
PLoS ONE, 11(10), e0164226-e0164226 (2016)
The endosomal protein endotubin is required for enterocyte differentiation
Cox CM, et al.
Cellular and molecular gastroenterology and hepatology, 5(2), 145-156 (2018)
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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