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Merck
모든 사진(1)

주요 문서

B3679

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

동의어(들):

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

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크기 선택

100 ML
₩364,690

₩364,690


재고 있음세부사항



크기 선택

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100 ML
₩364,690

About This Item

UNSPSC 코드:
12352204
NACRES:
NA.83

₩364,690


재고 있음세부사항


제품명

BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate

Quality Level

양식

liquid

저장 온도

2-8°C

애플리케이션

BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
  • for the detection of secondary antibodies by immunoblot[1]
  • in ELISPOT assay[2]
  • in dot blot analysis[3]
  • in western blot to visualize protein bands on nitrocellulose membrane[4]

생화학적/생리학적 작용

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.[4]

주의사항

Stable at least one year at 2-8 °C.

물리적 형태

Ready-to-use.

법적 정보

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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