A9776

α-Actinin from chicken gizzard

~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension

• CAS Number: 11003-00-2
• MDL number: MFCD00130473
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: chicken gizzard
• 분석: ~80% α-actinin basis (SDS-PAGE)
• 형태: ammonium sulfate suspension
• 분자량: 100 kDa
• UniProt 수납 번호: P05094; P12814; P20111; Q7TPR4; Q90734; Q9Z1P2
• 저장 온도: 2-8°C
• 유전자 정보: chicken ... ACTN2(396263) , ACTN4(396024) , RCJMB04_23c5(373918) ; human ... ACTN1(87) ; mouse ... ACTN1(109711) ; rat ... ACTN1(81634)

설명

일반 설명

α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.

애플리케이션

α-Actinin from chicken gizzard has been used:

• as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
• in in vitro motility experiments to test its effect on actin filament movement
• to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies

생화학적/생리학적 작용

α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.

포장

Package size based on protein content

물리적 형태

Suspension in 2 M (NH₄)₂SO₄ containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride

제조 메모

Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.

Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)