추천 제품
생물학적 소스
goat
결합
peroxidase conjugate
항체 형태
affinity isolated antibody
항체 생산 유형
secondary antibodies
클론
polyclonal
형태
buffered aqueous solution
기술
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
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일반 설명
Goat polyclonal Mouse IgG (Fc specific)–Peroxidase antibody is determined against normal mouse serum, mouse IgG (whole molecule), the Fc fragment of mouse IgG and the Fab fragment of mouse IgG, the conjugate is specific for mouse IgG and shows no reaction with the Fab fragment of mouse IgG. The conjugate only shows reactivity with mouse IgG (whole molecule) and the Fc fragment of mouse IgG, when tested in ELISA. The conjugate shows no reaction with the Fab fragment of mouse IgG, human IgG, IgA, IgM, or rat IgG in ELISA.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.
애플리케이션
Anti-Mouse IgG (Fc specific)-Peroxidase antibody has been used:
- in immunoblotting
- in immunohistochemistry
- in enzyme linked immunosorbent assay (ELISA)
- in agar block precipitin titration
- in western blotting
- in assessment of in vitro poly(ADP-ribose)polymerase (PARP) activity
Indirect ELISA was performed on sera from mice immunized to SV40-Tag using HRP-conjugated goat anti-mouse Fc specific IgG. Antibody was used at a 1:1000 dilution for 30 minutes at 37 degrees.
Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
생화학적/생리학적 작용
IgG antibodies help in neutralizing the effects caused by viruses and toxins.
IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
기타 정보
Antibody adsorbed with human IgG and rat serum proteins.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
제조 메모
Adsorbed to reduce background staining with human or rat samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
법적 정보
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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