A9205

Bovine Serum Albumin solution

30% in saline, fatty acid free, aseptically filled

• 동의어(들): Albumin solution from bovine serum, BSA solution
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 무균: aseptically filled
• 분석: 28-32% protein basis (biuret)
• 양식: solution
• 정제법: cold ethanol fractionation
• 미포함: stabilizer or preservative
• 기원: USA origin
• 농도: 28-32% protein (biuret); 30% in saline
• free fatty acid content: ≤0.01%
• 기술: ELISA: suitable; electrophoresis: suitable; western blot: suitable
• 불순물: ≤0.01% free fatty acid
• UniProt 수납 번호: P02769
• 외래 활성: BT virus, none detected; VSV virus, none detected
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.[1]

애플리케이션

Bovine Serum Albumin solution has been used:

• as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells[2]
• as a component of hepatocyte recovery medium[3]
• as a component of fusion and activation buffers for in vitro reconstructed sheep embryos[4]

생화학적/생리학적 작용

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis.[5] It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation.[6] Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry.[1] BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.[7]

제조 메모

Initial fractionation by cold alcohol precipitation

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)