A6781
Acetate Kinase from Bacillus stearothermophilus
lyophilized powder, 400-1,200 units/mg solid
동의어(들):
ATP:Acetate phosphotransferase, Acetate Kinase Bacillus stearothermophilus
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제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
eCl@ss:
32160410
MDL number:
Specific activity:
400-1,200 units/mg solid
Biological source:
bacterial (Bacillus stearothermophilus)
biological source
bacterial (Bacillus stearothermophilus)
Quality Segment
form
lyophilized powder
specific activity
400-1,200 units/mg solid
storage temp.
2-8°C
Application
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
Biochem/physiol Actions
Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 % α-helix and 43 % unordered structure.
Involved in the metabolism of propanoate, pyruvate and taurine.
Physical form
Contains Tris-HCl buffer.
Other Notes
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
저장 등급
11 - Combustible Solids