The milligram quantity of this product will vary from lot to lot. The vial content is dependent upon the protein content and enzymatic activity. The minimum protein content will be 70% and the minimum activity will be 2 units per milligram protein. As an example, a batch that reports a protein content of 100% and an activity of 2 units per milligram protein will have a vial content of 2.5 mg for the 5 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a6778#product-documentation
A6778
Angiotensin Converting Enzyme from rabbit lung
≥2.0 units/mg protein (modified Warburg-Christian)
동의어(들):
ACE, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
보기 변경
| 여러분께/SKU | 재고 정보 | 가격 (VAT 별도) |
|---|---|---|
0.1 unit | 오늘 배송 예정재고 수량 조회Seoul Warehouse South Korea | ₩180,900 |
0.25 unit | 오늘 배송 예정재고 수량 조회Seoul Warehouse South Korea | ₩350,100 |
1 unit | 오늘 배송 예정재고 수량 조회Seoul Warehouse South Korea | ₩957,500 |
2 units | 구입 가능 여부는 고객센터에 문의하십시오. | ₩1,957,600 |
5 units | 구입 가능 여부는 고객센터에 문의하십시오. | ₩4,027,300 |
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
eCl@ss:
42010109
NACRES:
NA.32
MDL number:
Specific activity:
≥2.0 units/mg protein (modified Warburg-Christian)
form
lyophilized powder
Quality Segment
specific activity
≥2.0 units/mg protein (modified Warburg-Christian)
shipped in
dry ice
storage temp.
−20°C
General description
The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure. Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril. Currently, developing inhibitory peptides from natural food sources, or phenolic compounds from plant sources to inhibit ACE is underway. ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.
Application
Angiotensin converting enzyme from rabbit lung has been used:
- for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)
- to measure the ACE inhibition by litchi pericarp and cooked chicken breast using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC respectively
- in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.
Biochem/physiol Actions
Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.
Analysis Note
May contain traces of sodium chloride.
Other Notes
One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.
1 of 1
이 품목 | |||
|---|---|---|---|
| specific activity ≥2.0 units/mg protein (modified Warburg-Christian) | specific activity ≥10 units/mg protein (Bradford) | specific activity ≥10 U/mg | specific activity ≥1000000 U/mg |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in ambient |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100, 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How many grams per 1 U?
1 답변-
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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What is the effect of bleomycin on Angiotensin Converting Enzyme (ACE) activity in vivo?
1 답변-
Bleomycin causes a marked reduction in rabbit lung ACE activity. Reference: Cancer Research, 40, 3621-3626, (1980).
도움이 되었습니까?
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Are there any other known inhibitors of rabbit lung Angiotensin Converting Enzyme (ACE), Product A6778?
1 답변-
Idrapril, the prototype of a new class of angiotensin converting enzyme (ACE) inhibitors, competitively inhibits, with nanomolar apparent Ki, the hydrolysis of hippuryl-glycyl-glycine by rabbit lung ACE. Kinetic Ki and kon and koff values were 470 pM, 3.0±1.5×106 M-1sec-1 and 1.4±0.3×10-3 sec-1, respectively. Reference: Biochemical Pharmacology, 45(6), 1358-1362 (1993).
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What is the medical significance of Angiotensin Converting Enzyme (ACE), Product A6778?
1 답변-
Angiotensin-converting enzyme (ACE) produces angiotensin II, a substance that affects the cardiovascular system in numerous ways, including narrowing of blood vessels. This narrowing can cause high blood pressure and cause the heart to work harder. ACE inhibitors treat a variety of conditions, such as high blood pressure, scleroderma and migraines. (Mayo Clinic)
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