B5525

Bromophenol Blue sodium salt

• 동의어(들): 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• 실험식(Hill 표기법): C₁₉H₉Br₄NaO₅S
• CAS Number: 34725-61-6
• Molecular Weight: 691.94
• Beilstein: 6995701
• EC Number: 252-170-2
• MDL number: MFCD00013793
• UNSPSC 코드: 12171500
• PubChem Substance ID: 57653941
• NACRES: NA.52

속성

• 양식: powder
• 기술: electrophoresis: suitable
• solubility: water: soluble
• 적합성: suitable for nucleic acid electrophoresis
• 저장 온도: room temp
• SMILES string: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• InChI key: TWKDJMRHSLWRFI-UHFFFAOYSA-M

설명

일반 설명

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

애플리케이션

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

재구성

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)