추천 제품
분석
≥80.0% (degree of coupling)
양식
solid
solubility
DMF: 1 mg/mL, clear
형광
λex 480 nm; λem 610-630 nm in PBS, pH 7.4
저장 온도
−20°C
일반 설명
Abberior STAR 470SX is the latest development of long-Stokes-Shift dyes for STED microscopy. The dye can be excited from 450 to 480nm. It can substitute dyes like Chromeo™ 494. For STED, a depletion wavelength ~750 nm is recommended. It is therefore well suited for 2-color STED imaging as implemented in the Leica TCS STED Ti:Sa microscope.
Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system.
Key Features
Absorption Maximum, λmax: 475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30′400 M-1cm-1 (MeOH),
22′700 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.69
Correction Factor, CF280 = ε280/max: 0.47
Fluorescence Maximum, λfl: 609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.80 (EtOH)
Fluorescence Lifetime, τ: 3.9 (EtOH)
Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system.
Key Features
- Designed for STED microscopy at ~750 nm
- Long Stokes′ shift (>130 nm) for 2-color applications
- Tested in the Leica TCS STED Ti:Sa 2-color system
Absorption Maximum, λmax: 475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30′400 M-1cm-1 (MeOH),
22′700 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.69
Correction Factor, CF280 = ε280/max: 0.47
Fluorescence Maximum, λfl: 609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.80 (EtOH)
Fluorescence Lifetime, τ: 3.9 (EtOH)
애플리케이션
Abberior® STAR 470SX goat anti-rabbit antibody has been used for STED (stimulated emission depletion) microscopy in Caco-2 cells.
적합성
Designed and tested for fluorescent super-resolution microscopy
법적 정보
Chromeo is a trademark of Active Motif Chromeon GmbH
abberior is a registered trademark of Abberior GmbH
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
Thommie Karlsson et al.
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Quorum sensing (QS) signaling allows bacteria to control gene expression once a critical population density is achieved. The Gram-negative human pathogen Pseudomonas aeruginosa uses N-acylhomoserine lactones (AHL) as QS signals, which coordinate the production of virulence factors and biofilms. These
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to
Beitraege zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung.
Abbe, E.
Archiv fur Mikroskopische Anatomie, 9, 413-420 (1873)
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