93363

Trizma^® hydrochloride

BioUltra, for molecular biology, ≥99.0% (AT)

• 동의어(들): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• EC Number: 214-684-5
• MDL number: MFCD00012590
• UNSPSC 코드: 12161700
• PubChem Substance ID: 57653541
• NACRES: NA.25

속성

• Grade: Molecular Biology; for molecular biology
• 제품 라인: BioUltra
• 분석: ≥99.0% (AT)
• 양식: powder or crystals
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• 무기 잔류물: ≤0.2% (as SO₄)
• 손실: ≤0.2% loss on drying, 110 °C
• pH: 2.5-4.0 (25 °C, 4 M in H₂O)
• 유용한 pH 범위: 7.0-9.0
• pKa(25 °C): 8.1
• mp: 150-152 °C
• solubility: H₂O: 4 M at 20 °C, clear, colorless
• 음이온 미량물: sulfate (SO₄^2-): ≤50 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 4 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.12; λ: 280 nm A_max: 0.08
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

설명

애플리케이션

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

기타 정보

Component of extraction buffer e.g. phenol extraction of DNA or RNAcitation; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGEcitation.

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Component of extraction buffer e.g. phenol extraction of DNA or RNA[1]; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE[2].

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves