75259

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, ≥98% (NMR)

• 동의어(들): TCEP
• 실험식(Hill 표기법): C₉H₁₅O₆P · HCl
• CAS Number: 51805-45-9
• Molecular Weight: 286.65
• Beilstein: 3724376
• MDL number: MFCD00145469
• UNSPSC 코드: 12352128
• PubChem Substance ID: 329766294
• NACRES: NA.25

속성

• 제품 라인: BioUltra
• 분석: ≥98% (NMR)
• 형태: crystals
• 반응 적합성: reagent type: reductant
• 손실: ≤1.0% loss on drying
• pH: 1.2-1.5
• solubility: H₂O: 1 M, clear, colorless (no residue)
• 음이온 미량물: sulfate (SO₄^2-): ≤50 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.05 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.03
• 저장 온도: 2-8°C
• SMILES string: Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

설명

일반 설명

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution. It readily and stoichiometrically reduces disulfides with high specificity. TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.

애플리케이션

Tris(2-carboxyethyl)phosphine hydrochloride has been used:

• as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA
• in reducing sulfhydryl groups to obtain total thiol fraction
• to reduce protein samples

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H314
• 예방조치 성명서: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 1