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Merck
모든 사진(1)

주요 문서

2851

Sigma-Aldrich

Citrate Phosphate Buffer Concentrate

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About This Item

UNSPSC 코드:
41116124
NACRES:
NA.47
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양식

solution

IVD

for in vitro diagnostic use

응용 분야

hematology
histology

배송 상태

wet ice

저장 온도

2-8°C

애플리케이션


  • Poly(sulfobetaine) versus poly(ethylene glycol) based copolymer modified polyurethane catheters for antifouling: This study examines the use of ethanol in the synthesis of polymers designed to reduce biological fouling on medical devices, highlighting its role in advanced healthcare materials research (Cao et al., 2024).

  • Green synthesis of polyimide by using an ethanol solvothermal method for aqueous zinc batteries: Ethanol is used as a green solvent in the innovative synthesis of polyimide structures, emphasizing its importance in sustainable material production and energy storage solutions (Zhao et al., 2024).

  • Modulation of gut microbiota with probiotics as a strategy to counteract endogenous and exogenous neurotoxicity: Research explores the indirect applications of ethanol in the solvent extraction processes used for studying neuroprotective strategies in medical research (Skalny et al., 2024).

  • Superselective Ablative Chemo-ethanol Embolization for Recurrent Single Hepatocellular Carcinoma: A Six-Month Outcome Analysis: This clinical study utilizes ethanol directly in therapeutic interventions, demonstrating its critical use in oncological treatments to improve patient outcomes (Lee et al., 2024).

  • Self-Assembly Reinforced Alginate Fibers for Enhanced Strength, Toughness, and Bone Regeneration: Ethanol is employed in the processing of novel biomaterials designed for medical applications, showcasing its utility in the development of regenerative medicine technologies (Cui et al., 2024).

성분

Sodium citrate, 0.7 mol/L, and sodium phosphate, 0.6 mol/L

면책조항

Sales restrictions may apply

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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질문

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  1. Are adult red blood cells failing to properly elute hemoglobin after incubation in the Citrate Phosphate Buffer working solution? Could the pH of the Citrate Phosphate Buffer be too high? Additionally, is it feasible to increase the amount of agitation to aid in eluting the adult hemoglobin?

    1 답변
    1. To elute adult hemoglobin, pure/undenatured 80% alcohol is necessary. The use of histological or reagent grade alcohol may result in inadequate elution.

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  2. Is it advisable to switch to the published procedure, which requires a 5-minute incubation, considering the change in cell appearance after immersing the slides for 2 minutes in the buffer?

    1 답변
    1. The recommended procedure for a 5-minute incubation in 1X Citrate Phosphate Buffer solution should be followed, with adjustments made based on the degree of staining observed with the slides. The slides should be agitated at 1 and 3 minutes, and the user should determine the amount of agitation required based on their observations. The degree of agitation may need to be adjusted to address issues such as hard-to-observe ghost cells or an excess of adult hemoglobin.

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