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Merck
모든 사진(1)

주요 문서

211-500

Sigma-Aldrich

Endothelial Cell Growth Medium (500 ml)

동의어(들):

Primary Cell Culture Media

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모든 사진(1)

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71
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양식

liquid

Quality Level

100

유통기한

6 mo.

제조업체/상표

Cell Applications, Inc

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

All-in-one ready-to-use media
Endothelial Cell Growth Medium (500 ml) is an all-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies.

애플리케이션

Endothelial Cell Growth Medium (500 ml) has been used to culture human aortic endothelial cells[1][2] and human umbilical vein endothelial cells.[3][4]

품질

Each lot was tested for the ability to support plating, spreading and proliferation

제조 메모

contained in protocol

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
36705
36305

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문서 라이브러리 방문

Yang Li et al.
Life sciences, 232, 116590-116590 (2019-06-23)
Endothelial cell (EC) apoptosis is fundamental for the pathophysiology of atherosclerosis, in which microRNAs (miRNAs) emerge as critical regulators. miR-122 has been shown to regulate the apoptosis of various cell types, however, whether miR-122 is associated with atherosclerosis and EC
Angel Tan et al.
Biomaterials science, 7(8), 3460-3470 (2019-07-04)
In the research field of nanostructured systems for biomedical applications, increasing attention has been paid to using biomimetic, dynamic cellular models to adequately predict their bio-nano behaviours. This work specifically evaluates the biointeractions of nanostructured lipid-based particles (cubosomes) with human
Ekramy Elmorsy et al.
Toxicology in vitro : an international journal published in association with BIBRA, 44, 154-163 (2017-07-18)
Antidepressants are commonly employed for the treatment of major depressive disorders and other psychiatric conditions. We investigated the relatively acute cytotoxic effects of three commonly prescribed antidepressants: fluoxetine, sertraline, and clomipramine on rat primary blood brain barrier endothelial cells over
Zheng Wei et al.
Cellular signalling, 44, 118-126 (2018-01-18)
Thromboangiitis obliterans (TAO) (also known as Buerger's disease) is an inflammatory vascular disease that predominantly affects small- and medium-sized blood vessels of extremities. Endothelial cells play critical roles in the initiation and progression of this disease, but the underlying mechanisms
Jaya Sriram et al.
International journal of environmental research and public health, 15(10) (2018-10-24)
Airborne particulate matter (PM) exposure remains the leading environmental risk factor for disease globally. Interventions to mitigate the adverse effects of PM are required, since there is no discernible threshold for its effects, and exposure reduction approaches are limited. The
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문서

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

프로토콜

Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol

Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

질문

1–4 / 4 질문  

  1. Does the medium contain VEGF? If so, can you specify the concentration?

    1 답변

    1. This medium does not contain VEGF.

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  2. If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?

    1 답변

    1. This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.

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  3. Can you please provide the full composition of the medium with the concentrations?

    1 답변

    1. This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.

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  4. Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.

    1 답변

    1. This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.

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