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Merck
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문서

PPB010

Sigma-Aldrich

Tris EDTA Buffer, pH 8.0

pHast Pack, powder, 10X concentrate

동의어(들):

10X TE buffer

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About This Item

UNSPSC 코드:
12161700
NACRES:
NA.25

제품 라인

pHast Pack

형태

powder

저장 조건

(Tightly closed. Dry)

불순물

≤5 ppm Heavy Metals (lead)

색상

white

pH

7.9—8.1

solubility

soluble, clear, colorless

양이온 미량물

Fe: <10 ppm

외래 활성

DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected

저장 온도

room temp

일반 설명

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

애플리케이션

TE buffer is intended for use in:
  • Nucleic acid gel electrophoresis
  • storage and dilution of DNA and RNA
  • DNA automated sequencing
  • Immunohistochemistry

특징 및 장점

  • Fast and easy Ready-to-use buffer
  • Minimizes effort - no need to weigh and adjust pH
  • Saves time and eliminates exposure to hazardous chemicals
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

포장

Foil pouch

재구성

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

저장 및 안정성

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

픽토그램

Health hazard

신호어

Warning

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

STOT RE 2 Inhalation

표적 기관

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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