E9379

Stains-All

~95% purity, powder

• 동의어(들): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• 실험식(Hill 표기법): C₃₀H₂₇BrN₂S₂
• CAS Number: 7423-31-6
• Molecular Weight: 559.58
• Beilstein: 3865213
• EC Number: 231-047-7
• MDL number: MFCD00078788
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24894694
• NACRES: NA.47

속성

• 제품명: Stains-All, ~95%
• 분석: ~95%
• Quality Level: 200
• 양식: powder
• 기술: microbe id | staining: suitable
• solubility: chloroform: 40 mg (PLUS 4.0 ml)
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: 2-8°C
• SMILES string: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• InChI key: MPBRYMWMMKKRGC-UHFFFAOYSA-M

설명

애플리케이션

Stains-All has been used for the staining of gel made for size determination of hyaluronan.[1][2] It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.[3]

생화학적/생리학적 작용

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange.[4] It also stains RNA (bluish purple) and DNA (blue).[5]

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

주의사항

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)