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Merck
모든 사진(1)

문서

909815

Sigma-Aldrich

4-Azido-L-homoalanine hydrochloride

≥98%

동의어(들):

(S)-2-Amino-4-azidobutanoic acid hydrochloride, 4-Azido-L-homoalanine HCl, 4-Azido-L-homoalanine hydrochloride, H-L-Aha-OH*HCl

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모든 사진(1)

About This Item

실험식(Hill 표기법):
C4H8N4O2 · HCl
CAS Number:
942518-29-8
Molecular Weight:
180.59
UNSPSC 코드:
12352209

분석

≥98%

형태

powder

반응 적합성

reaction type: solution phase peptide synthesis

재고 정보

available only in USA

저장 온도

2-8°C

InChI

1S/C4H8N4O2.ClH/c5-3(4(9)10)1-2-7-8-6;/h3H,1-2,5H2,(H,9,10);1H/t3-;/m0./s1

InChI key

MHHYJRIDKLZZEO-DFWYDOINSA-N

애플리케이션

The in vivo incorporation of clickable unnatural amino acids such as 4-Azido-L-homoalanine with unique reactivity at a defined postition is used for functionalization of proteins. The azide functionalities in the protein can then be modified with almost any alkyne bearing molecule by Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). Copper-free alternatives with strained internal alkynes are also available (SPAAC). Some examples enabled with this technique are protein PEGylation, masking with sugars, and the attachment to antibodies.

픽토그램

Flame
GHS02

신호어

Danger

유해 및 위험 성명서

H242

Hazard Classifications

Self-react. C

Storage Class Code

5.2 - Organic peroxides and self-reacting hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
MKCK5743

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문서 라이브러리 방문

Jigang Wang et al.
Nature protocols, 12(2), 279-288 (2017-01-13)
At present, several assays that use radioisotope labeling to quantify the degradation of long-lived proteins have been developed to measure autophagic flux. Here, we describe a nonradioactive pulse-chase protocol using L-azidohomoalanine (AHA) labeling to quantify long-lived protein degradation during autophagy.
Milena Ullrich et al.
Nature protocols, 9(9), 2237-2255 (2014-08-29)
In this protocol we describe the incorporation of bio-orthogonal amino acids as a versatile method for visualizing and identifying de novo-synthesized proteins in the roundworm Caenorhabditis elegans. This protocol contains directions on implementing three complementary types of analysis: 'click chemistry'

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