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Merck
모든 사진(1)

문서

49940

Millipore

ONPG Disks

suitable for microbiology

동의어(들):

β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks

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About This Item

UNSPSC 코드:
41171621
NACRES:
NA.85

Agency

according to GB 4789.4-2016

제품 라인

BioChemika

유통기한

limited shelf life, expiry date on the label

기술

microbe id | specific enzyme detection: suitable

응용 분야

environmental
food and beverages

microbiology

배송 상태

wet ice

저장 온도

2-8°C

적합성

Escherichia coli
bacteria

일반 설명

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

포장

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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Leann Clark et al.
Microbiology (Reading, England), 157(Pt 7), 2072-2083 (2011-04-16)
Most studies on Salmonella enterica serovar Typhimurium infection focus on strains ATCC SL1344 or NTCC 12023 (ATCC 14028). We have compared the abilities of these strains to induce membrane ruffles and invade epithelial cells. S. Typhimurium strain 12023 is less

문서

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

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