Product 318337 is not function tested during quality control testing for use in either the sperm chromatin structure assay (SCSA) or the sperm DNA structure assay. The product is sold for Research Use Only. Quality Control testing includes testing for appearance, infrared spectrum, extinction coefficients at 3 different wavelengths, carbon, nitrogen, purity by HPLC, zinc, solubility, and water content. The literature shows that Acridine Orange is used for differential staining of DNA and RNA and a wide range of other uses. The website does list various uses for product 318337. The list however is not all inclusive of the uses for which the dye can be used. It is up to the customer to determine if the dye is acceptable for their intended use(s).
318337
Acridine Orange hydrochloride hydrate
≥98% (HPLC)
동의어(들):
3,6-Bis(dimethylamino)acridine hydrochloride
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모든 사진(4)
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250 MG
₩58,363
1 G
₩205,944
About This Item
실험식(Hill 표기법):
C17H19N3 · HCl · xH2O
CAS Number:
Molecular Weight:
301.81 (anhydrous basis)
색상 지수 번호:
46005
Beilstein:
3734978
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
Quality Level
분석
≥98% (HPLC)
양식
powder
기술
microbe id | staining: suitable
색상
orange
mp
284-287 °C (lit.)
solubility
H2O: 0.1%, clear, orange to red
λmax
492 nm
ε (흡광계수)
≥14,000 at 227-233 nm in water at 0.005 g/L
≥35000 at 265-271 nm in water at 0.005 g/L
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
InChI
1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
InChI key
JUAAUEXLEKAEQC-UHFFFAOYSA-N
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애플리케이션
A rigorously purified zinc-free material suitable for use in flow cytometry.
Acridine Orange hydrochloride hydrate dye has been used:
Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.
생화학적/생리학적 작용
Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA.[3] Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.[4]
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Muta. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
F. W. D. Rost
Fluorescence Microscopy, 2 (1992)
Viriditoxin regulates apoptosis and autophagy via mitotic catastrophe and microtubule formation in human prostate cancer cells.
Kundu S, et.al.
International Journal of Oncology, 45, 2331-2340 (2014)
Autophagy inhibition uncovers the neurotoxic action of the antipsychotic drug olanzapine
Vucicevic L
Autophagy, 10, 2362-2378 (2014)
Autophagy-dependent senescence in response to DNA damage and chronic apoptotic stress
Singh K, et.al.
Autophagy, 8, 236-251 (2012)
Michael M Fisher et al.
International journal of radiation oncology, biology, physics, 107(4), 793-803 (2020-04-17)
We examined the capacity of the pan-fibroblast growth factor receptor (FGFR) inhibitor AZD4547 to augment radiation response across a panel of head and neck squamous cell carcinoma (HNSCC) cell lines and xenografts. FGFR1, FGFR2, and FGFR3 RNA in situ hybridization
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