XTG9-RO

X-tremeGENE^™ 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

• UNSPSC 코드: 41106502
• NACRES: NA.55

속성

• Grade: for molecular biology
• Quality Level: 100
• 양식: liquid (aqueous solution)
• 사용: mL (suitable for 165 transfections)
• 포장: pkg of 0.4 mL (06365779001); pkg of 1.0 mL (06365787001); pkg of 5 × 1.0 mL (06365809001)
• 제조업체/상표: Roche
• 기술: transfection: suitable
• 저장 온도: 2-8°C

설명

일반 설명

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

애플리케이션

X-tremeGENE^™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE^™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:

• Expression of recombinant proteins for functional analysis.
• Physiological studies of metabolic pathways.[1][2]
• Analysis of regulatory sequences using reporter gene assays.[3]
• Gene expression assays.[3][4]
• Cancer research studies.[5]
• Target evaluation.

특징 및 장점

• Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
• Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
• Avoid time-consuming optimization procedures in commonly used cell lines.

품질

Each lot of X-tremeGENE^™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE^™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

물리적 형태

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

X-tremeGENE is a trademark of Roche

안전성 정보

• 픽토그램: GHS02,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H225 - H319
• 예방조치 성명서: P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point (°F): 334.4 °F
• Flash Point (°C): 168 °C