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EAGLETMMRO

Roche

EagleTaq Universal Master Mix (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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About This Item

UNSPSC 코드:
41106300
NACRES:
NA.55

사용

sufficient for 100 reactions
 sufficient for 500 reactions
 sufficient for 5000 reactions

Quality Level

100

특징

dNTPs included
hotstart

제조업체/상표

Roche

포장

pkg of 100 x 20 μL reactions (07260288190)
pkg of 500 x 20 μL reactions (07260296190)
pkg of 5000 x 20 μL reactions (07249926190)

기술

RT-qPCR: suitable
qPCR: suitable

입력

purified DNA

검출 방법

probe-based

일반 설명

2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.

The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

애플리케이션

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).

특징 및 장점

  • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
  • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
  • The hot start properties allow reaction setup at ambient temperature.
  • With this robust reagent, any PCR protocol optimization is minimized.

성분

Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

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시험 성적서(COA)

Lot/Batch Number

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이미 열람한 고객

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ReadyMix™ Taq PCR Reaction Mix with MgCl2

Sigma-Aldrich

P4600

ReadyMix™ Taq PCR Reaction Mix

Uracil-DNA Glycosylase, heat-labile recombinant from marine bacterium BMTU 3346

Roche

UDGHL-RO

Uracil-DNA Glycosylase, heat-labile

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문서

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

관련 콘텐츠

PCR 애플리케이션

중합효소연쇄반응(PCR)은 핵산 분자를 증폭하는 기술이며 RT-PCR, hot start PCR, end point PCR을 포함한 여러 애플리케이션에서 일반적으로 활용됩니다.

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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