DUTP-RO

dUTP

PCR Grade, sodium salt

• 동의어(들): 2′-Deoxyuridine 5′-triphosphate sodium salt solution, dUTP sodium salt
• 실험식(Hill 표기법): C₉H₁₅N₂O₁₄P₃ · xNa⁺
• CAS 번호: 94736-09-1
• Molecular Weight: 468.14 (free acid basis)
• NACRES: NA.21
• PubChem Substance ID: 329798931
• UNSPSC Code: 41106305
• MDL number: MFCD01630759
• Assay: 99% (dNTP)
• Form: solution
• Storage temp.: −20°C

속성

• assay: 99% (dNTP)
• form: solution
• usage: sufficient for 125,000 standard reactions (03732720001), sufficient for 31,250 standard reactions (11969056001), sufficient for 6,250 standard reactions (11934554001)
• packaging: pkg of 1,250 μL (11969056001 [100 mM]), pkg of 4 × 1,250 μL (03732720001 [4x100 mM]), pkg of 250 μL (11934554001 [100 mM])
• manufacturer/tradename: Roche
• concentration: <0.9% (dNDP), 100 mM
• technique(s): whole genome amplification: suitable
• storage temp.: −20°C
• SMILES string: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O14P3.Na.H/c12-5-3-8(11-2-1-7(13)10-9(11)14)23-6(5)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,12H,3-4H2,(H,18,19)(H,20,21)(H,10,13,14)(H2,15,16,17);;
• InChI key: FVFWWPFKJNTHIQ-UHFFFAOYSA-N

설명

General description

dUTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Application

dUTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Avoid carryover contamination between PCRs to eliminate a source of false positives.

Physical form

solution of dUTP water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (30 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 30 μl dUTP to 40 μl Water,PCR grade).

Analysis Note

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Other Notes

For general laboratory use.

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

안전성 정보

• 저장 등급: 12 - Non Combustible Liquids
• 인화점 (F): does not flash
• 인화점 (°C): does not flash
• 무슨 일이 일어나고 있는 걸까?: WGK 3