추천 제품
분석
>98% (NMR)
Quality Level
사용
sufficient for 50 blots (10 cm x 10 cm each)
포장
kit of 1 (5 components)
제조업체/상표
Roche
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
기술
hybridization: suitable
환경친화적 대안 카테고리
, Aligned
저장 온도
−20°C
일반 설명
DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
애플리케이션
DIG Luminescent Detection Kit has been used for highly sensitive and specific detection of DIG-labeled nucleic acids in:
- critical commercial assays
- Southern hybridization
- northern analysis
- plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.
포장
1 kit containing 5 components.
규격
Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.
제조 메모
Working concentration: 0.5mg/ml for flow cytometry
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
Note: Store protected from light.
- Storage conditions (working solution): Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
- Blocking Reagent (bottle 4): in solution at 2 to 8°C
- CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
- Immunological detection 1.5 hours
- Signal detection 5 to 30 minute
Note: Store protected from light.
기타 정보
For life science research only. Not for use in diagnostic procedures.
키트 구성품 전용
제품 번호
설명
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer
- Anti-digoxigenin-AP antibody, Fab fragments 750 U/ml
- Blocking Reagent
- CSPD 11.6 mg/ml
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
does not flashNot applicable
Flash Point (°C)
does not flashNot applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Karl Dichtl et al.
Molecular microbiology, 95(3), 458-471 (2014-11-27)
Echinocandins inhibit β-1,3-glucan synthesis and are one of the few antimycotic drug classes effective against Aspergillus spp. In this study, we characterized the β-1,3-glucan synthase Fks1 of Aspergillus fumigatus, the putative target of echinocandins. Data obtained with a conditional mutant
Dongqing Huang et al.
Genetics, 203(1), 353-368 (2016-03-28)
In response to replication stress, a phospho-signaling cascade is activated and required for coordination of DNA repair and replication of damaged templates (intra-S-phase checkpoint) . How phospho-signaling coordinates the DNA replication stress response is largely unknown. We employed state-of-the-art liquid
DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.
Huang D, et al.
Genetics, 203(1), 353-368 (2016)
Jes-Niels Boeckel et al.
Circulation research, 117(10), 884-890 (2015-09-18)
Circular RNAs (circRNAs) are noncoding RNAs generated by back splicing. Back splicing has been considered a rare event, but recent studies suggest that circRNAs are widely expressed. However, the expression, regulation, and function of circRNAs in vascular cells is still
Circ-ZNF609 is a circular RNA that can be translated and functions in myogenesis.
Legnini I, et al.
Molecular Cell, 66(1), 22-37 (2017)
문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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