추천 제품
사용
sufficient for 25 labeling reactions
sufficient for 50 blots
Quality Level
제조업체/상표
Roche
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
환경친화적 대안 카테고리
, Aligned
저장 온도
−20°C
일반 설명
DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers.
Contents:
Contents:
- Unlabeled Control DNA 1, 100 μg/ml
- Unlabeled Control DNA 2, 200 μg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA, 5.2 μg/ml
- 10x Hexanucleotide Mix
- 10x dNTP Labeling Mixture
- Klenow Enzyme, Labeling grade, 2 U/μl
- Anti-digoxigenin-AP-conjugate, 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
특이성
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
애플리케이션
DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Dot blots
- Colony and plaque screening
포장
1 kit containing 10 components.
제조 메모
Working concentration: Dilution of Antibody 1:5,000
기타 정보
For life science research only. Not for use in diagnostic procedures.
키트 구성품 전용
제품 번호
설명
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
모두 보기 (10)
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
does not flashNot applicable
Flash Point (°C)
does not flashNot applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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