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추천 제품
생물학적 소스
rat tail
Quality Level
무균
sterile
양식
lyophilized (clear, colorless solution after reconstitution)
포장
pkg of 30 mg
제조업체/상표
Roche
기술
cell culture | mammalian: suitable
UniProt 수납 번호
저장 온도
2-8°C
유전자 정보
rat ... Col1a1(29393)
일반 설명
Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens are mainly present in the extracellular matrix.[1] Collagen type I is abundantly present in mammals.[2] Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.[3]
특이성
Active on most mammalian cells.
애플리케이션
생화학적/생리학적 작용
특징 및 장점
Contents
Lyophilizate, cell culture grade, 3 vials of 10mg
Lyophilizate, cell culture grade, 3 vials of 10mg
규격
Biological activity: Tested for the promotion of adherence of HUV-EC cells.
제조 메모
Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
재구성
IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
Identification and analysis of a novel dimerization domain shared by various members of JNK scaffold proteins.
Cohen-Katsenelson K, et al.
The Journal of Biological Chemistry (2013)
Lodish H
Molecular and Cellular Biology (2000)
Collagen: Structure and Mechanics (2008)
Roman G Szafran et al.
Molecules (Basel, Switzerland), 26(21) (2021-11-14)
In this study, we thoroughly analyzed molecular gradient generation, its stability over time, and linearity in our high-throughput drug screening microfluidic assay (HTS). These parameters greatly affect the precision and accuracy of the device's analytical protocol. As part of the
Calcium-based nanoparticles accelerate skin wound healing.
Kawai K, et al.
PLoS ONE, 6(11), e27106-e27106 (2011)
문서
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
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