11093070910

Biotin-16-dUTP

• 동의어(들): Bio-16-dUTP tetralithium salt, Biotin-labeled Uridines, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine 5′-triphosphate
• 실험식(Hill 표기법): C₃₂H₅₂N₇O₁₈P₃S
• CAS Number: 136632-31-0
• Molecular Weight: 947.78
• MDL number: MFCD32011849
• UNSPSC 코드: 41116100

속성

• 양식: solution
• Quality Level: 100
• 포장: pkg of 50 μL (50 nmol; 1mM)
• 제조업체/상표: Roche
• 색상: colorless
• solubility: water: miscible
• 저장 온도: −20°C

설명

일반 설명

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.[1]Biotin-16-dUTP is used to produce biotinylated DNA probes that are commonly used to detect specific DNA and RNA sequences in different methods like Southern and Northern blotting, in situ hybridization, and sandwich assays.[2]

애플리케이션

Biotin-16-dUTP has been used:

• For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.[3] Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.[4]
• as a substrate for terminal transferase, DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase, and reverse transcriptase (e.g., Transcriptor). The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling
• In fluorescence in situ hybridization (FISH)[5]

생화학적/생리학적 작용

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones.[1] It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.[6]

품질

Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).

분석 메모

Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax₂₄₁: E = 10.700;
Emax₂₉₂: E = 7.000;
Emax₂₆₅: E = 4.400;
A_292/265 : 1.6.

기타 정보

Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate- Biotin Luminescence Detection Kit.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash