08039852001

Carboxypeptidase B, recombinant

solution, ~150 units/mg protein (at +25°C with hippuryl-L-arginine as the substrate), optimum pH 7.0-9.0

• EC 번호: 3.4.17.2 (BRENDA, IUBMB)
• NACRES: NA.21
• UNSPSC Code: 12352204
• Specific activity: ~150 units/mg protein (at +25°C with hippuryl-L-arginine as the substrate)

속성

• form: solution
• specific activity: ~150 units/mg protein (at +25°C with hippuryl-L-arginine as the substrate)
• shelf life: A decrease in activity of approx. 10% may occur within 6 months.
• mol wt: 34.6 kDa
• packaging: pkg of 1 mL (2.8 mg)
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• optimum pH: 7.0-9.0
• storage temp.: −20°C

설명

Application

Peptidyl-L-lysine (-L-arginine) hydrolase

Use Carboxypeptidase B for the sequence analysis of proteins by successive cleavage of basic amino acids from the C-terminus of proteins.

Biochem/physiol Actions

Carboxypeptidase B is a Zn²⁺-metalloprotease, catalyzing the hydrolysis of the basic amino acids L-Lys and L-Arg from the C-terminal position in polypeptides.

Analysis Note

Contaminants: Contaminants expressed as percentage of carboxypeptidase B activity.
Carboxypeptidase A ≤2%
Trypsin (with Chymotrypsin as substrate) ≤0.005 U/mg

Other Notes

For life science research use only. Not for use in diagnostic precedures.

안전성 정보

• 저장 등급: 12 - Non Combustible Liquids
• 인화점 (F): does not flash
• 인화점 (°C): does not flash