Unfortunately, the cell line originator does not designate gender in the original patent application or the original published paper. The author simply states that the line was derived from non-ischemic ventricular sample from an ischemic explanted human heart following IRB approval. Please see the link below to review the original publication:
https://www.sciencedirect.com/science/article/pii/S0022282805000763?via%3Dihub
추천 제품
제품명
AC16 Human Cardiomyocyte Cell Line, AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.
생물학적 소스
human
Quality Level
기술
cell culture | mammalian: suitable
배송 상태
dry ice
일반 설명
AC16 Human Cardiomyocyte Cell Line is a proliferating cell line that was derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA [1][2]. After selection in uridine-free medium to remove unfused fibroblasts, the resulting fused cells were further subcloned and subsequently screened for the presence of SV40 large T-ag, β-myosin heavy chain (βMHC) and connexin-43 (CX-43) [1].
AC16 cells are proliferating and differentiation of the cells may be controlled by altering culture conditions and by silencing the expression of SV40 T-Ag [1]. The cells can be used to study cardiac gene expression and function, during normal development and in pathological conditions at the cellular, organellar and molecular levels.
AC16 cells are proliferating and differentiation of the cells may be controlled by altering culture conditions and by silencing the expression of SV40 T-Ag [1]. The cells can be used to study cardiac gene expression and function, during normal development and in pathological conditions at the cellular, organellar and molecular levels.
세포주 설명
Cardiomyocyte Cells
애플리케이션
Research Category
Cardiac
Cardiovascular Disease
Toxicity
Cardiac
Cardiovascular Disease
Toxicity
This product is available for sale to academic institutions or not-for-profit entities for research use. For information on commercial licensing please contact [email protected].
품질
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
저장 및 안정성
AC16 Human Cardiomyocyte Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Vikram Thakur et al.
Biomolecules, 11(4) (2021-05-01)
Myocardial fibrosis is one of the major complications of long-term diabetes. Hyperglycemia induced cardiomyocyte atrophy is a frequent pathophysiological indicator of diabetic heart. The objective of this study was to investigate the cardioprotective effect of glycyrrhizin (GLC) on myocardial damage
P Litzkas et al.
Molecular and cellular biology, 4(11), 2549-2552 (1984-11-01)
A method for fusion of protoplasts bearing amplified plasmids and human diploid fibroblasts or other cell types in suspension is described. Transient expression of plasmid-encoded proteins occurs in up to 50% of the human cells, as demonstrated for simian virus
Vikram Thakur et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Diabetes is a major risk factor for cardiovascular diseases, especially cardiomyopathy, a condition in which the smooth muscles of the heart become thick and rigid, affecting the functioning of cardiomyocytes, the contractile cells of the heart. Uncontrolled elevated glucose levels
Lucia Scisciola et al.
Frontiers in pharmacology, 13, 868365-868365 (2022-06-04)
The cellular mechanisms involved in myocardial ischemia/reperfusion injury (I/R) pathogenesis are complex but attributable to reactive oxygen species (ROS) production. ROS produced by coronary endothelial cells, blood cells (e.g., leukocytes and platelets), and cardiac myocytes have the potential to damage
Gayathri Narasimhan et al.
Clinical and experimental pharmacology & physiology, 46(8), 743-751 (2019-05-08)
Obg-like ATPase 1 (OLA1) that possesses both GTP and ATP hydrolyzing activities has been shown to be involved in translational regulation of cancer cell growth and survival. Also, GSK3β signalling has been implicated in cardiac development and disease. However, the
관련 콘텐츠
Learn how to use our cell culture tested, ready-to-use MilliShot™ single dose antibiotic solutions, conveniently packaged in one-time use vials.
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I would like ti know What sex are the cells
1 답변-
도움이 되었습니까?
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Who invented this cell line?
1 답변-
AC16 Human Cardiomyocyte Cell Line is a proliferating cell line that was derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA. After selection in uridine-free medium to remove unfused fibroblasts, the resulting fused cells were further subcloned and subsequently screened for the presence of SV40 large T-ag, β-myosin heavy chain (βMHC) and connexin-43 (CX-43) .
AC16 cells are proliferating and differentiation of the cells may be controlled by altering culture conditions and by silencing the expression of SV40 T-Ag The cells can be used to study cardiac gene expression and function, during normal development and in pathological conditions at the cellular, organellar and molecular levels.
Please see this article for information on cell-line invention: https://www.sigmaaldrich.com/tech-docs/paper/1383711
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