PMEF-CFL

EmbryoMax^® Primary Mouse Embryonic Fibroblasts

• 동의어(들): CF-1 MEF Feeder Cells, CF-1 MEFs, CF-1 PMEFs, CF1 Mouse Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
• UNSPSC 코드: 41106509
• eCl@ss: 32011203
• NACRES: NA.75

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 제조업체/상표: Specialty Media; EmbryoMax^®
• 기술: cell culture | stem cell: suitable
• 입력: sample type: human embryonic stem cell(s); sample type: mouse embryonic stem cell(s); sample type primary embryotic fibroblasts (PMEFs); sample type induced pluripotent stem cell(s)
• 배송 상태: liquid nitrogen

설명

일반 설명

Primary Mouse Embryo Fibroblasts, Strain CF1, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use.

Plating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

세포주 설명

Primary Mouse Embryo Fibroblasts

포장

5-6x10⁶ ea

물리적 형태

The cells are provided frozen into individual vials, containing approximately 5-6 x 10⁶ fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3. See ES Stem Cell Manual for complete culture and medias for PMEF cells.

저장 및 안정성

After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

법적 정보

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash