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MRCF0R100

Millipore

Microcon® DNA Fast Flow Centrifugal Filters

sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding)

동의어(들):

Centrifuge concentrator

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크기 선택

100 EA
₩859,604

₩859,604

예상 입고일2025년 4월 16일세부사항

벌크 견적 요청
모든 사진(3)

크기 선택

보기 변경
100 EA
₩859,604

About This Item

UNSPSC 코드:
41104916
eCl@ss:
36100101
NACRES:
NB.24

₩859,604

예상 입고일2025년 4월 16일세부사항

벌크 견적 요청

material

Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)

Quality Level

400

무균

non-sterile

특징

holdup volume> 10 μL

포장

pkg of 100 ea

제조업체/상표

Microcon®

파라미터

0.5 mL sample volume

기술

DNA purification: suitable
RNA purification: suitable

길이

4.50 cm (1.8 in.)

직경

12.3 mm

여과 면적

0.32 cm2

작업 용적

0.5 mL

배송 상태

ambient

관련 카테고리

일반 설명

Microcon® centrifugal filters provide efficient concentration, desalting, or buffer exchange of aqueous biological samples ranging in volume from 10–500 μL. The Microcon® DNA fast flow filter is optimized for the concentration and recovery of genomic DNA with SDS buffer.

애플리케이션

Microcon DNA Fast Flow Centrifugal Filter Unit with Ultracel membrane has been used to extract regular organic DNA to compare DNA yields from alternative extraction methods.[1] It has also been used to filter the digested DNA for electron microscopic analysis of genomic DNA.[2]

특징 및 장점

Microcon® centrifugal filters have several benefits:
  • high recovery for small volumes with reverse spin
  • low-binding Ultracel® membrane
  • fast processing
  • yield concentration factors <20X for consistent reproducibility

결합

Replaces: 42413

법적 정보

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Bacillus spp. Bacteriophage and the Dairy Environment
Adamso KL and McKillip JL
Liaoning Chemical Industry, 23(2), 4-4 (2019)
Matteo Berti et al.
Nature communications, 11(1), 3531-3531 (2020-07-17)
Homologous recombination (HR) factors were recently implicated in DNA replication fork remodeling and protection. While maintaining genome stability, HR-mediated fork remodeling promotes cancer chemoresistance, by as-yet elusive mechanisms. Five HR cofactors - the RAD51 paralogs RAD51B, RAD51C, RAD51D, XRCC2 and

관련 콘텐츠

Microcon® Centrifugal Filters Applications Guidelines & Specifications

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.

질문

1–3 / 3 질문  

  1. Can I use a Microcon DNA fast flow device to isolate DNA from a crude cell lysis preparation such a freeze thaw in TE buffer or a chelex 100 extraction?

    1 답변

    1. Yes, a Microcon DNA fast flow device can be used to isolate DNA from a crude cell lysis preparation. However, lysates typically contain heavy particulate contamination, so clarification spins may be necessary to remove particulates before attempting ultrafiltration.

      도움이 되었습니까?

  2. The user guide indicates you can't autoclave these microcon, but can you sterilize them in a UV Crosslinker?

    1 답변

    1. The Microcon devices are non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent Preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter device.

      2.Sanitization Protocol:

      A. Fill the Microcon device to its published capacity with filter-sterilized 70% ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).

      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.

      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).

      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.

      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.

      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.

      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.

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  3. Has the color of the Microcon DNA Fast Flow become brighter blue?

    1 답변

    1. Yes, the color of the device has changed. A new blue colorant and clear resin have replaced the original blue reservoir resin, which has been discontinued. Both old and new resin are HP2 Polycarbonate. The functionality of the product has not changed.

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