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Merck
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MHSTCMAG-70K

Millipore

MILLIPLEX® Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

동의어(들):

Luminex® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay

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About This Item

UNSPSC 코드:
12161503
eCl@ss:
32161000
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
Design And Price Your Kit

Quality Level

200

종 반응성

mouse

제조업체/상표

Milliplex®

assay range

accuracy: 82-102%
sensitivity: 0.26-11.14 pg/mL
standard curve range: 0.10-400 pg/mL
(IL-4)
standard curve range: 0.49-2,000 pg/mL
(IFNγ)
standard curve range: 0.49-2,000 pg/mL
(IL-17A)
standard curve range: 0.49-2,000 pg/mL
(IL-7)
standard curve range: 0.49-2,000 pg/mL
(TNFα)
standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1)
standard curve range: 0.98-4,000 pg/mL
(IL-2)
standard curve range: 0.98-4,000 pg/mL
(IL-5)
standard curve range: 0.98-4,000 pg/mL
(IL-6)
standard curve range: 1.34-5,500 pg/mL
(IL-10)
standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70))
standard curve range: 3.17-13,000 pg/mL
(LIX)
standard curve range: 3.42-14.000 pg/mL
(IL-13)
standard curve range: 3.91-16,000 pg/mL
(IL-1α)
standard curve range: 3.91-16,000 pg/mL
(IL-1β)
standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2)
standard curve range: 6.1-25,000 pg/mL
(GM-CSF)
standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2)
inter-assay cv: <15%
intra-assay cv: <10%

기술

multiplexing: suitable

검출 방법

fluorometric (Luminex xMAP)

배송 상태

ambient

관련 카테고리

일반 설명

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Panel Type: Cytokines/Chemokines

특이성

Cross Reactivty
There is no detectable cross-reactivity within the panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

애플리케이션

  • Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α
  • Recommended Sample type: serum, plasma, tissue culture or other media
  • Recommended Sample dilution: neat or 1:2 diluted serum or plasma
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Immune Response Metabolic Disorders Chronic Inflammatory Disease Cardiovascular Disease

특징 및 장점

Design your multiplex kit by choosing available analytes within this panel.

저장 및 안정성

Recommended storage for kit components is 2 - 8°C.

기타 정보

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see protocol for sensitivity of individual analytes.

법적 정보

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

신호어

Danger

유해 및 위험 성명서

H302 + H312 + H332,H317,H318,H373,H411

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

표적 기관

Respiratory Tract

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

David K Harrison et al.
Transplantation, 105(3), 540-549 (2020-09-06)
Organ transplantation is life-saving and continued investigations into immunologic mechanisms that drive organ rejection are needed to improve immunosuppression therapies and prevent graft failure. DNA-dependent protein kinase catalytic subunit, DNA dependent-protein kinase catalytic subunit (DNA-PKcs), is a critical component of
Sha-Sha Yi et al.
Frontiers in psychiatry, 13, 864481-864481 (2022-05-17)
Increasing evidence indicates that inflammatory responses may influence brain neurochemical pathways, inducing depressive-like behaviors. Ultrasound stimulation (US) is a promising non-invasive treatment for neuropsychiatric diseases. We investigated whether US can suppress inflammation and improve depressive-like behaviors. Mice were intraperitoneally injected
Robert A Kazmierczak et al.
Oncotarget, 11(44), 3943-3958 (2020-11-21)
Conventional cancer chemotherapies are not fully efficacious and do not target tumors, leading to significant treatment-related morbidities. A number of genetically attenuated cancer-targeting bacteria are being developed to safely target tumors in vivo. Here we report the toxicological, tumor-targeting, and
Irene Martínez de Toda et al.
International journal of molecular sciences, 18(7) (2017-07-25)
The decrease in the proliferative response of lymphocytes is one of the most evident among the age-related changes of the immune system. This has been linked to a higher risk of mortality in both humans and experimental animals. However, long-lived
Anna M Klawonn et al.
Immunity, 54(2), 225-234 (2021-01-22)
Microglia are activated in many neurological diseases and have been suggested to play an important role in the development of affective disorders including major depression. To investigate how microglial signaling regulates mood, we used bidirectional chemogenetic manipulations of microglial activity
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관련 콘텐츠

Evaluation of MILLIPLEX® Mouse High Sensitivity T Cell Panel

The MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panel is a powerful tool for cytokine profiling in biological samples. Uncover details on how this panel was evaluated for studying low-level cytokine expression and also quantifying multiple cytokine secretion levels simultaneously.

질문

1–2 / 2 질문  

  1. When running the Mouse High Sensitivity Cytokine Panel with tissue lysates prepared in a Lysis Buffer from BioRad that is compatible with their BioPlex kits, should I use Serum Matrix to prepare the standards, or should I use the buffer in which my samples are prepared?

    1 답변

    1. It is recommended to use the same buffer in which the samples are prepared wherever the protocol specifies the use of Serum Matrix. This ensures consistent assay conditions in the standard, QC, and sample wells.

      It's important to note that the kit has not been validated with this buffer, so there is a possibility that some QCs may read out of range when using this buffer. In such cases, it may be necessary to establish their own ranges using this buffer.

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  2. Before purchasing the Mouse MHSTCMAG-70K kit, I have a couple of questions regarding the assessment of three cytokines (TNF alpha, IL-6, and IL-1b) in plasma and brain extracts.

    1 답변

    1. I have provided some information that should assist you with your project. There is a protocol available that describes how to create a brain homogenate, including the appropriate concentration. Additionally, I have included a research paper on rat brain with a comprehensive methods section; however, we do not have one available for mouse. You will also find our general guide to buffers. Determining the total protein to add for the assay will depend on the specific experimental conditions. Nevertheless, our cytokine kits offer a wide standard curve range, and considering the sample type, using a more concentrated sample in the assay is likely the best approach. Although we do not use tissue samples in our kits, we have not established the appropriate sample concentration for this. Drawing from our cell signaling kits, which utilize cell lysates, it is recommended to use between 1 and 25 ug of total protein per well in the assay, which may serve as a good starting point for you. In terms of a protease inhibitor, we recommend using the Sigma protease inhibitor cocktail P2714, which should be hydrated in 100 mls of water per bottle and is a 100X stock.

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