콘텐츠로 건너뛰기
Merck
모든 사진(2)

주요 문서

모든 문서 보기

MCYTOMAG-70K-PMX

Millipore

MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

동의어(들):

Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Immunoassay

로그인조직 및 계약 가격 보기
모든 사진(2)

About This Item

UNSPSC 코드:
12161503
eCl@ss:
32161000
NACRES:
NA.47
가격 및 재고 정보를 현재 이용할 수 없음

Quality Level

200

종 반응성

mouse

제조업체/상표

Milliplex®

assay range

accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL

기술

multiplexing: suitable

호환성

configured for Premixed

검출 방법

fluorometric (Luminex xMAP)

배송 상태

wet ice

관련 카테고리

일반 설명

To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:

  • More flexible plate and plate washer options
  • Improved performance with turbid serum/plasma samples
  • Assay results equivalent to non- beads
  • Automated washing avoids many problems associated with vacuum filtration washing


MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

Panel Type: Cytokines/Chemokines

애플리케이션

  • Analytes: G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, MCP-1, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α

기타 정보

Sensitivity: Refer to kit protocol for sensitivities for individual cytokines.

법적 정보

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

신호어

Danger

유해 및 위험 성명서

H302 + H312 + H332,H317,H318,H373,H411

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

표적 기관

Respiratory Tract

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Elena A Govorkova et al.
Antimicrobial agents and chemotherapy, 59(3), 1495-1504 (2014-12-24)
Compounds that target the cellular factors essential for influenza virus replication represent an innovative approach to antiviral therapy. Sp2CBMTD is a genetically engineered multivalent protein that masks sialic acid-containing cellular receptors on the respiratory epithelium, which are recognized by influenza
Dana M Hardbower et al.
Amino acids, 48(10), 2375-2388 (2016-04-15)
We reported that arginase 2 (ARG2) deletion results in increased gastritis and decreased bacterial burden during Helicobacter pylori infection in mice. Our studies implicated a potential role for inducible nitric oxide (NO) synthase (NOS2), as Arg2 (-/-) mice exhibited increased
Kshipra Singh et al.
American journal of physiology. Gastrointestinal and liver physiology, 305(3), G225-G240 (2013-05-25)
L-Arginine (L-Arg) is a semiessential amino acid that has altered availability in human ulcerative colitis (UC), a form of inflammatory bowel disease, and is beneficial in murine colitis induced by dextran sulfate sodium (DSS), a model with similarity to UC.
Ruchi Srivastava et al.
The Journal of general virology, 96(Pt 6), 1347-1357 (2015-02-11)
West Nile virus (WNV), an important global human pathogen, targets neurons to cause lethal encephalitis, primarily in elderly and immunocompromised patients. Currently, there are no approved therapeutic agents or vaccines to treat WNV encephalitis. Recent studies have suggested that inflammation
Christine I Wooddell et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 21(5), 973-985 (2013-02-27)
RNA interference (RNAi)-based therapeutics have the potential to treat chronic hepatitis B virus (HBV) infection in a fundamentally different manner than current therapies. Using RNAi, it is possible to knock down expression of viral RNAs including the pregenomic RNA from
모든 관련된 서류 보기

질문

1–2 / 2 질문  

  1. It seems that the sample wells are experiencing low bead counts while the standard and control wells are unaffected. This issue is limited to 1 or 2 cytokines, while the rest show normal counts. It doesn't appear to be related to the instrument, bead loss, or inadequate bead mixing. What could be the possible reason for this discrepancy?

    1 답변

    1. To reduce the occurrence of sample-related bead aggregation, the following steps can be taken:
      •Spin samples at 10,000 x g for 10 minutes immediately before preparing sample dilutions or loading samples into the plate.
      •Resuspend the plate in Wash Buffer instead of Sheath or Drive Fluid. The plate must be read within a couple of hours, and the buffer should be switched to Sheath or Drive Fluid for longer storage.
      •Dilute samples at a ratio of 1:2 or 1:4.
      •Perform more wash cycles after the primary incubation step of the assay to remove substances that cause bead aggregation.

      도움이 되었습니까?

  2. Does the addition of 25 µL of Assay Buffer to the sample well count as a dilution when using a Milliplex kit that does not require a sample dilution prior to loading the plate?

    1 답변

    1. If the sample is not diluted prior to loading the plate, and the same volume of standard and sample are loaded, then there is no need to apply a sample dilution factor.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.