MABT821

Anti-Integrin beta1 (CD29) Antibody, clone mAb13

clone mAb13, from rat

• 동의어(들): Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: mAb13, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 기술: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_596867
• UniProt 수납 번호: P05556
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGB1(3688)

설명

일반 설명

Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrin beta-1 is a single-pass type I membrane protein that can associate with one of the multiple alpha subunits to serve as a receptor for fibronectin and other extracellular matrix proteins. Integrin beta-1 integrin recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin beta-1 have been reported that are produced by alternative splicing. Integrin beta-1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin beta-1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).

특이성

Clone mAb13 specifically detects beta 1 subunit of human integrin. It targets an epitope within 12 amino acids from the N-terminal half.

면역원

Fibronectin receptor (alpha V beta 1) purified from human placenta.

애플리케이션

Anti-Integrin beta1 (CD29), clone mAb13, Cat. No. MABT821, is a rat monoclonal antibody that detects integrin beta-1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition studies.

Inhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).

Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).

Research Category
Cell Structure

품질

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Integrin beta1 (CD29) in A431 cells.

표적 설명

88.42 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash